High performance liquid chromatography was used in order to detect cheese whey in samples of raw milk preserved with Bronopol®. Six samples were collected and divided in 45 aliquots of 40mL. From these, 15 were used as control and stored frozen, 15 were added with Bronopol® and stored at 7°C, and the other 15 were added with Bronopol® and stored at 30°C. In all groups, five levels of cheese whey addition (0, 2, 5, 10, and 20%) were tested. The samples were submitted to high performance liquid chromatography on the 2 nd, 4 th, and 8 th days of storage. A completely random design was used, following the factorial scheme (5×3×3) and the results were compared through the non-parametric Kruskal-Wallis test. There was no difference among the treatments (P>0.05), which allows the conclusion that raw milk preserved with Bronopol® may be used for the determination of cheese whey addition in milk through high performance liquid chromatography.
Lasmar, M. M., Leite, M. O., Fonseca, L. M., Souza, M. R., Cerqueira, M. M. O. P., Penna, C. F. A. M., … Ferreira, J. M. (2011). Detection of cheese whey in raw milk preserved with bronopol® through high performance liquid chromatography. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 63(6), 1553–1558. https://doi.org/10.1590/S0102-09352011000600037