Aging and exposure to sunlight are two major factors in the deterioration of skin function. In this study, thirty-six fixed human skin samples from sun-exposed and unexposed areas from young and old individuals were used to evaluate the localization of oxidative stress according to levels and distribution of 8-hydroxy-2′-deoxyguanosine and N ε- (carboxymethyl)lysine in samples using immunohistochemistry. In the epidermis of the young, negligible amounts of 8-hydroxy-2′-deoxyguanosine and N ε-(carboxymethyl)lysine were detected in unexposed areas, whereas nuclear 8-hydroxy-2′-deoxyguanosine and cytoplasmic N ε-(carboxymethyl)lysine were increased in the lower epidermis in sun-exposed areas. In contrast, the aged presented prominent nuclear 8-hydroxy-2′-deoxyguanosine and nuclear N ε-(carboxymethyl) lysine in the epidermis of unexposed areas, concomitant with dermal increase in N ε-(carboxymethyl)lysine. However, the immunostaining of 8-hydroxy-2′-deoxyguanosine and N ε-(carboxymethyl)lysine revealed a decrease in the epidermis of sun-exposed areas in the aged. These results suggest an age-dependent difference in the adaptation and protective mechanisms of the epidermis against sunlight-associated oxidative stress, thus necessitating distinct standards for evaluation in each age group. Further investigation is warranted to elucidate underlying molecular mechanisms. ©2011 JCBN.
CITATION STYLE
Toyokuni, S., Hirao, A., Wada, T., Nagai, R., Date, A., Yoshii, T., … Kawada, A. (2011). Age- and sun exposure-dependent differences in 8-hydroxy-2′- deoxyguanosine and N ε-(carboxymethyl)lysine in human epidermis. Journal of Clinical Biochemistry and Nutrition, 49(2), 121–124. https://doi.org/10.3164/jcbn.11-05
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