High proliferative potential colony-forming cell heterogeneity identified using counterflow centrifugal elutriation

23Citations
Citations of this article
6Readers
Mendeley users who have this article in their library.

Abstract

Murine high proliferative potential colony-forming cells (HPP-CFC) are known to be heterogenous with respect to proliferative capacity and in vitro responsiveness to hematopoietic growth factors. We have separated HPP-CFC into several subpopulations using counterflow centrifugal elutriation. Although HPP-CFC were identified in all of the elutriated fractions of both C3H/HeJ and C57BI/6J bone marrow cells, the distribution of HPP-CFC as well as of colony-forming units - granulocyte-macrophage (CFU-GM) in each fraction differed between these two strains of inbred mice. Six subsets of HPP-CFC were resolved that differed in growth factor responsiveness. A low-density HPP-CFC subpopulation was isolated that was distinct from day-12 spleen colony-forming units (CFU-S12), CFU-GM, and bone marrow stromal cells. This unique subpopulation of HPP-CFC is rare (3% to 9% of total HPP-CFC), appears to be lymphocyte-like in morphology, and behaves the most primitive of the HPP-CFC subsets by requiring multiple hematopoietic growth factors for optimal in vitro cloning. Further characterization of this subpopulation of HPP-CFC will determine the position of these cells in the HPP-CFC heirarchy. © 1993 by The American Society of Hematology.

Cite

CITATION STYLE

APA

Yoder, M. C., Du, X. X., & Williams, D. A. (1993). High proliferative potential colony-forming cell heterogeneity identified using counterflow centrifugal elutriation. Blood, 82(2), 385–391. https://doi.org/10.1182/blood.v82.2.385.bloodjournal822385

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free