The development of a multicellular organism is accompanied by cell differentiation. In fact, many biological processes have cell specificity, such that distinct cell types respond differently to endogenous or environmental cues. To obtain cell-specific gene expression profiles, translating ribosome affinity purification (TRAP) has been developed to label polysomes containing translating mRNAs in genetically defined cell types. Here, we describe the immunopurification of epitope-labeled polysomes and associated RNAs from target cell types. TRAP has the additional advantage of obtaining only translating mRNAs, which are a better proxy to the proteome than a standard mRNA preparation. © Springer Science+Business Media, New York 2014.
CITATION STYLE
Wang, Y., & Jiao, Y. (2014). Translating ribosome affinity purification (TRAP) for cell-specific translation profiling in developing flowers. Methods in Molecular Biology, 1110, 323–328. https://doi.org/10.1007/978-1-4614-9408-9_18
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