We have identified in rabbits two hepatic forms of T669 peptide kinases that are very strongly activated after systemic injection with the inflammatory cytokine interleukin 1 (IL-1). The T669 peptide contains a major phosphorylation site of the epidermal growth factor receptor, threonine 699 and is a substrate for mitogen-activated protein (MAP) kinases. The kinases were purified to homogeneity and corresponded to 50- and 55-kD proteins on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Amino acid sequencing of 12 tryptic peptides of both kinases identified them as p54 MAP kinase α. This kinase belongs to the novel family of stress-activated protein kinases. This is the first evidence of IL-1 activating a specific protein kinase in vivo.
Mendeley helps you to discover research relevant for your work.
CITATION STYLE
Kracht, M., Truong, O., Totty, N. F., Shiroo, M., & Saklatvala, J. (1994). Interleukin 1 α activates two forms of p54α mitogen-activated protein kinase in rabbit liver. Journal of Experimental Medicine, 180(6), 2017–2025. https://doi.org/10.1084/jem.180.6.2017