Evaluation of TBARS, SOD and GPx in saliva during pregnancy of murrah buffaloes maintained under isohyet conditions

Abstract

The objective of the present study was to assess the oxidative status in saliva samples during pregnancy by measuring salivary antioxidant enzymes and oxidative stress marker in Murrah buffaloes. We considered saliva as an alternative biological sample because of its non-invasiveness and also from farmer’s perspective. In our study 20 saliva samples each ten were collected from non-pregnant and pregnant Murrah buffaloes maintained under agro climatic regions receiving equal rain fall and laid on equal elevation to avoid geospatial differences. The mean saliva TBARS concentration was significantly (P< 0.05) higher (2.59±0.31 µmoles/L) whereas SOD and GPx levels were significantly (P < 0.05) lower during pregnancy when compared to non-pregnant Murrah buffaloes. In our study, a significant increase in concentration of TBARS in saliva of pregnant Murrah buffaloes was observed whereas the activity of antioxidant enzymes SOD and GPx were significantly decreased in saliva during pregnancy.