Hydrogen peroxide (H2 O2) produced from mitochondria is intimately involved in human health and disease but is challenging to selectively monitor inside living systems. The fluorescent probe MitoPY1 provides a practical tool for imaging mitochondrial H2 O2 and has been demonstrated to function in a variety of diverse cell types. In this chapter, we describe the synthetic preparation of the small-molecule probe MitoPY1, methods for validating this probe in vitro and in live cells, and an example procedure for measuring mitochondrial H2 O2 in a cell culture model of Parkinson’s disease.
CITATION STYLE
Lippert, A. R., Dickinson, B. C., & New, E. J. (2015). Imaging mitochondrial hydrogen peroxide in living cells. Methods in Molecular Biology, 1264, 213–243. https://doi.org/10.1007/978-1-4939-2257-4_21
Mendeley helps you to discover research relevant for your work.