A Simple Rp-Hplc Method for Simultaneous Estimation of Paracetamol and Metoclopramide. HCl inTablet Dosage Form

Abstract

A simple, rapid, specific, precise and accurate reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the simultaneous estimation of Paracetamol and Metoclopramide.HCl in tablet dosage form. The chromatographic separation was achieved on AM Chemteq C18 (250mm x 4.6mm, 5µ) column at a detector wavelength of 220nm using an isocratic mobile phase comprising a mixture of Methanol: 0.5% Ammonium dihydrogen orthophosphate buffer (pH: 3) (35:65v/v) pumped at a flow rate of 1.0ml/min. Paracetamol and Metoclopramide.HCl eluted at the retention times of 4.35 and 7.5min respectively. The method was validated with respect to parameters such as specificity, linearity, precision, accuracy, robustness, limit of detection, limit of quantification, system suitability. The proposed RP-HPLC method can be used for the estimation of these drugs in tablet dosage form.