Mass spectrometry (MS) provides analyte identification over a wide molar-mass range. However, particularly in the case of complex matrices, this ability is often enhanced by the use of pre-MS separation steps. A separation, prototype technique for the "gentle" fractionation of large/ultralarge analytes, from proteins to whole cells, is here described to reduce complexity and maintain native characteristics of the sample before MS analysis. It is based on flow field-flow fractionation, and it employs a micro-volume fractionation channel made of a ca. 20 cm hollow-fiber membrane of sub-millimeter section. The key advantages of this technique lie in the low volume and low-cost of the channel, which makes it suitable to a disposable usage. Fractionation performance and instrumental simplicity make it an interesting methodology for in-batch or on-line pre-MS treatment of such samples. © 2011 Springer Science+Business Media B.V.
CITATION STYLE
Reschiglian, P., Zattoni, A., Rambaldi, D. C., Roda, A., & Hee Moon, M. (2011). Hollow-fiber flow field-flow fractionation for mass spectrometry: From proteins to whole bacteria. In NATO Science for Peace and Security Series A: Chemistry and Biology (pp. 13–36). Springer Verlag. https://doi.org/10.1007/978-90-481-9815-3_2
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