Effect of nitrate and ammonium nutrition of nonnodulated Phaseolus vulgaris L. On phosphoenolpyruvate carboxylase and pyruvate kinase activity

Abstract

Young bean plants (Phaseolus vulgaris L. var Saxa) were fed with 3.5 or 10 millimolar N in either the form of N0 3 - or NH 4 + after being grown on N-free nutrient solution for 8 days. The pH of the nutrient solutions was either 6 or 4. The cell sap pH and the extractable activities of phosphoenolpyruvate carboxylase and of pyruvate kinase from roots and primary leaves were measured over several days. The extractable activity of phosphoenolpyruvate carboxylase (based on soluble protein) from primary leaves increased with N0 3 - nutrition, whereas with NH 4 + nutrition and on N-free nutrient solution the activity remained at a low level. Phosphoenopyruvate carboxylase activity from the roots of NfV-fed plants at pH 4 was finally somewhat higher than from the roots of plants grown on N0 3 - at the same pH. There was no difference in activity from the root between the N treatments when pH in the nutrient solutions was 6. The extractable activity of pyruvate kinase from roots and primary leaves seemed not to be influenced by the N nutrition of the plants. The results are discussed in relation to the physiological function of both enzymes with special regard to the postulated functions of phospho­enolpyruvate carboxylase in C3 plants as an anaplerotic enzyme and as part of a cellular pH stat.