Increased plasmin and serine proteinase activity during flow-induced intimal atrophy in baboon PTFE grafts

54Citations
Citations of this article
26Readers
Mendeley users who have this article in their library.

Abstract

High blood flow causes intimal atrophy and loss of extracellular matrix in PTFE aortoiliac grafts. We have investigated whether matrix-degrading proteinases are altered in this baboon model of atrophy using zymography, western analysis, and a versican degradation assay. After four days of high flow, urokinase was increased and plasminogen activator inhibitor-1 was decreased in the intima. Plasminogen was increased after seven days. Pro-matrix metalloproteinase (MMP)-2, activated MMP-2, and proMMP-9 levels were modestly increased by high flow at 7 days, whereas MMP-3 and tissue inhibitor of metalloproteinases-1 were not altered. Extracts of 4-day high-flow intimas degraded more 35S-methionine-labeled versican than low-flow intimal extracts, and this activity was inhibited by AEBSF, a serine proteinase inhibitor, and a plasmin antibody. In contrast, this activity was not inhibited by the MMP inhibitor, BB-94 (Batimastat). These data suggest that serine proteinases, including plasmin, may be largely responsible for extracellular matrix degradation in this primate model of flow-induced intimal atrophy.

Cite

CITATION STYLE

APA

Kenagy, R. D., Fischer, J. W., Davies, M. G., Berceli, S. A., Hawkins, S. M., Wight, T. N., & Clowes, A. W. (2002). Increased plasmin and serine proteinase activity during flow-induced intimal atrophy in baboon PTFE grafts. Arteriosclerosis, Thrombosis, and Vascular Biology, 22(3), 400–404. https://doi.org/10.1161/hq0302.105376

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free