Isolation of prebiotic inulin from Gadung Aceh tuber (Dioscorea Hispida) using hydrolysis reaction

Abstract

The goal of this research to identifity inulin compounds in Aceh gadung tuber (Dioscorea Hispida) by using hydrolysis method with variations hydrolysis time 30 minutes; 60 minutes; 90 minutes and hydrolysis temperature 70°C; 80°C. Inulin is a group of carbohydrate that are prebiotic. Inulin is usually use a substitute for fat and sugar in low calorie food product. The hydrolysis method used can convert the carbohydrate from tuber starch to glucose and also convert it to simple sugar that is fructan. The analysis in this research is detected inulin function group by analysis Fourier Transform Infrared Spectroscopy (FTIR) and yield of crystal inulin. Isolation inulin used material and solvent (1:5) can obtained the best treatment of isolation inulin is operating condition at hydrolysis temperature 70°C by hydrolysis time 60 minutes, in this condition the yield of crystal inulin is 42%.