Recent technological advances have enabled visualization of the organization and dynamics of local chromatin structures; however, the global mechanisms by which chromatin organization modulates gene regulation are poorly understood. We designed and constructed a human artificial chromosome (HAC) vector that allows regulation of transgene expression and delivery of a gene expression platform into many vertebrate cell lines. This technology for manipulating a transgene using a HAC vector could be used in applied biology.
CITATION STYLE
Ikeno, M., & Suzuki, N. (2011). Construction and Use of a Bottom-Up HAC Vector for Transgene Expression. In Methods in Molecular Biology (Vol. 738, pp. 101–110). Humana Press Inc. https://doi.org/10.1007/978-1-61779-099-7_7
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