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Determining nucleosome position at individual loci after biotic stress using MNase-qPCR

Humana Press Inc., (2016), 357-372
DOI: 10.1007/978-1-4939-3356-3_29
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Abstract

Nucleosome occupancy in promoter and genic regions can severely influence the transcription levels. Few methods have been established to investigate the nucleosome occupancy along the DNA. In this chapter we describe a detailed protocol to analyze the nucleosome occupancy at a specific locus using MNase-pPCR.

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Kaster, M., & Laubinger, S. (2016). Determining nucleosome position at individual loci after biotic stress using MNase-qPCR. In Methods in Molecular Biology (Vol. 1398, pp. 357–372). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3356-3_29

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