Utilization of tofu wastewater and sugar industry by-products as a medium for the production of antifungal metabolites by Paecylomyces Marquand StrainTP4

Abstract

Paecilomyces Marquand strain TP4 is a saprophytic fungus isolated from soil taken from Tangkuban Perahu Mountain at West Java Province, Indonesia. This research aims to identify the growth profile of P. Marquand strain TP4 and the antifungal activity against Fusarium sp. The antifungal compound is produced by the liquid fermentation method using tofu wastewater and molasses media. The fermentation broth is extracted by liquid-liquid extraction using n-hexane, ethyl acetate and methanol solvents. The result shows that the optimum activity achieved after 120 hours of fermentation. The fermentation broth has antifungal activity against Fusarium sp., where the inhibitory diameter was 20 mm. The total flavonoid content and total phenolic content of freeze-dry fermentation culture were 0.4839 mg/g and 1.171 mg/g, respectively. Ethyl acetate extract shows the highest antifungal activity with MIC 31.25 ppm and MFC 125 ppm. The FTIR spectrum shows the OH, aliphatic CH, CC, aromatic C=C, CH and C-O. Characterization of an antifungal compound based on phytochemical test and FTIR determine to be a phenolic group.