Brain quantitative proteomics combining gelc-ms and isotope-coded protein labeling (ICPL)

Abstract

Proteomics has been revolutionized by the rapid advance of mass spectrometric instrumentations and techniques. Parallel methodologies for the quantifi cation of proteomes also evolved, including in vitro stable isotope labeling. Here, we present a protocol for employing isotope-coded protein labeling (ICPL) as part of a shotgun proteomics work flow denoting its advantages and disadvantages. This protocol is suitable to studying any proteome of interest, only requiring a specifi c sample preparation and protein identification. Given our expertise, descriptions here are centered on the study of brain disorders.