Cell-based microarrays are valuable platforms for the study of cytotoxicity and cellular microenvironment because they enable high-throughput screening of large sets of conditions at reduced reagent consumption. However, most of the described microarray technologies have been applied to two-dimensional cultures, which do not accurately emulate the in vivo three-dimensional (3D) cell–cell and cell–extracellular matrix interactions. Herein, we describe the methodology for production of alginate- and Matrigel-based 3-D cell microarrays for the study of mouse and human pluripotent stem cells on two different chip-based platforms. We further provide protocols for on-chip proliferation/viability analysis and the assessment of protein expression by immunofluorescence.
CITATION STYLE
Pascoal, J. F., Fernandes, T. G., Nierode, G. J., Diogo, M. M., Dordick, J. S., & Cabral, J. M. S. (2018). Three-dimensional cell-based microarrays: printing pluripotent stem cells into 3D microenvironments. In Methods in Molecular Biology (Vol. 1771, pp. 69–81). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7792-5_6
Mendeley helps you to discover research relevant for your work.