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Book Chapter

Localization of Proteins and Organelles Using Fluorescence Microscopy

  • Farre J
  • Shirahama-Noda K
  • Zhang L
  • et al.
DOI: 10.1007/978-1-59745-456-8_17
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Abstract

This chapter describes the different methods used for localization of proteins and organelles in Pichia pastoris. A series of plasmids and a modified immunofluorescence protocol for localization and co-localization of proteins and organelles are described. Also included are protocols for the labeling of different subcellular organelles with vital stains.

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APA

Farre, J.-C., Shirahama-Noda, K., Zhang, L., Booher, K., & Subramani, S. (2007). Localization of Proteins and Organelles Using Fluorescence Microscopy (pp. 239–249). https://doi.org/10.1007/978-1-59745-456-8_17

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