Regulation of gene expression by short oligonucleotides (antisense oligonucleotides), which can modulate RNA structures and inhibit subsequent associations with the translation machinery, is a potential approach for gene therapy. This chapter describes an alternative antisense strategy using guanine-tethered antisense oligonucleotides (G-ASs) to introduce a DNA-RNA heteroquadruplex structure at a designated sequence on RNA targets. The feasibility of using G-ASs to modulate RNA conformation may allow control of RNA function by inducing biologically important quadruplex structures. This approach to manipulate quadruplex structures using G-ASs may expand the strategies for regulating RNA structures and the functions of short oligonucleotide riboregulators. © Springer Science+Business Media New York 2014.
CITATION STYLE
Hagihara, M. (2014). Guanine-tethered antisense oligonucleotides as synthetic riboregulators. Methods in Molecular Biology, 1111, 197–207. https://doi.org/10.1007/978-1-62703-755-6_14
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