In vitro protective effect of mangiferin against glycated protein-iron chelate induced toxicity in human umbilical vein endothelial cells

Abstract

The aim of the present study was to evaluate the protective effect of mangiferin on Human Umbilical Vein Endothelial Cells (HUVEC) against glycated protein-iron chelate induced toxicity. HUVEC incubated in glycated protein either alone or combined with iron chelate showed a significant (p<0.001) elevation of Lipid Peroxidation (LPO) accompanied by depletion of Superoxide Dismutase (SOD), catalase, Glutathione Peroxidase (GPx) and Glutathione Reductase (GR), in addition to increased microsomal cytochrome c reductase and decreased glutathione S-Transferase (GST). Treatment of HUVEC with mangiferin at a concentration of 5 and 10 μg significantly decreased the level of LPO and altered antioxidants, cytochrome c reductase and GST levels to near normal in a dose dependent manner. Present results suggest that mangiferin have protective effect against glycated protein-iron chelate induced toxicity. © 2007 Asian Network for Scientific Information.