The roles of timosaponin in inhibiting adherence of fungus and enhancing function of phagocyte

Abstract

To understand the mechanisms of antifungal role and immunoloregulation about timosaponin, we adopt adhesion experiment that After pretreating the standard strains (ATCC14053, CMCCClc) of candida albicans with medicine of different concentration, added the human buccal epithelium cells and cultured, then calculated fungal count on each buccal epithelium which 100 cells were counted randomly in optical microscope. we adopted in vivo phagocytic experimentation on BALB/c mice that calculated phagocytic percentage and phagocytic index of phagocyte on Candida albicans. Results showed that there were the significant difference which value was P<0.001 compared experimental group with control group, the strength of inhibition was positively correlated with drug concentration. The mean of phagocytic percentage of phagocyte on ATCC14053 and CMCCC1c was 75.1% and 67.9%, which P value was P<0.01 and P<0.01, the mean of phagocytic index phagocyte on ATCC14053 and CMCCC1c was 2.03 and 1.53, which P value was P<0.01 and P<0.01. These results prompt that timosaponin can apparently inhibit the adherence of Candida allbicans to the human buccal epithelium cell and enhance phagocytosis of mice phagocyte on candida albicans. © 2012 Springer-Verlag GmbH.