Determination of enalapril maleate and atenolol in their pharmaceutical products and in biological fluids by flow-injection chemiluminescence

Abstract

A chemiluminescent method using flow injection (FI) was investigated for rapid and sensitive determination of enalapril maleate and atenolol, which are used in the treatment of hypertension. The method is based on the sensitizing effect of these drugs on the Ce(IV)-sulfite reaction. The different experimental parameters affecting the chemiluminescence (CL) intensity were carefully studied and incorporated into the procedure. The method permitted the determination of 0.01-3.0 μg mL-1 of enalapril maleate in bulk form with correlation coefficient r = 0.99993, lower limit of detection (LOD) 0.0025 μg mL-1 (S/N = 2) and lower limit of quantitation (LOQ) 0.01 μg mL-1. The linearity range of atenolol in bulk form was 0.01-2.0 μg mL-1 (r = 0.99989) with LOD of 0.0003 μg mL-1 (S/N = 2) and LOQ of 0.01 μg mL-1. In biological fluids the linearity range of enalapril maleate was 0.1-2.0 μg mL-1 in both urine and serum, and for atenolol the linearity range was 0.1-1.0 μg mL-1 in both urine and serum. The method was also applied to the determination of the drugs in their pharmaceutical preparations. © 2009 John Wiley & Sons, Ltd.