Ex vivo cultured neuronal networks emit in vivo-like spontaneous activity

16Citations
Citations of this article
43Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Spontaneous neuronal activity is present in virtually all brain regions, but neither its function nor spatiotemporal patterns are fully understood. Ex vivo organotypic slice cultures may offer an opportunity to investigate some aspects of spontaneous activity, because they self-restore their networks that collapsed during slicing procedures. In hippocampal networks, we compared the levels and patterns of in vivo spontaneous activity to those in acute and cultured slices. We found that the firing rates and excitatory synaptic activity in the in vivo hippocampus are more similar to those in slice cultures compared to acute slices. The soft confidence-weighted algorithm, a machine learning technique without human bias, also revealed that hippocampal slice cultures resemble the in vivo hippocampus in terms of the overall tendency of the parameters of spontaneous activity.

Cite

CITATION STYLE

APA

Okamoto, K., Ishikawa, T., Abe, R., Ishikawa, D., Kobayashi, C., Mizunuma, M., … Ikegaya, Y. (2014). Ex vivo cultured neuronal networks emit in vivo-like spontaneous activity. Journal of Physiological Sciences, 64(6), 421–431. https://doi.org/10.1007/s12576-014-0337-4

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free