Circ-0027599/PHDLA1 suppresses gastric cancer progression by sponging miR-101-3p.1

Abstract

Background: Pleckstrin homology-like domain family A member 1 (PHLDA1) is a tumor suppressor gene in gastric cancer, but its role regulated by circular RNAs (circRNAs) is not known. CircRNAs are important regulators in cancer growth and progression, however, the molecular roles of circRNAs in gastric cancer are rarely known. The study was aimed to investigate the role of circRNAs in regulating PHLDA1 expression in gastric cancer. Results: The circRNA expression profile in the gastric cancer tissues by circRNA microarray showed that hsa-circ-0027599 (circ-0027599) was significantly down-regulated in gastric cancer patients and cells when comparing with the controls. Circ-0027599 overexpression suppressed gastric cancer cell proliferation and metastasis. By using bioinformatics tools and luciferase reporter assays, circ-0027599 was verified as a sponge of miR-101-3p.1 (miR-101) and suppressed cancer cell survival and metastasis. It was also verified that PHLDA1 was regulated by circ-0027599 in gastric cancer cells. Conclusions: The study uncovered that PHLDA1 was regulated by circ-0027599/miR-101, which suppressed gastric cancer survival and metastasis in gastric cancer.