Outer Membrane Protein (OMP) Profiles of Brucella abortus Local Isolate by SDS-PAGE Procedure

Abstract

Brucella abortus is a Gram-negative, facultative intracellular bacteria of the genus Brucella that common cause of brucellosis in cattle. The Outer Membrane Protein (OMP) is an essential organelle of Gram-negative bacteria to fulfil several some many tasks and is crucial as a protective antigen. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a universal method for molecular weight separation and analysis of proteins. This study aimed to determine the profile Outer Membrane Protein (OMP) band composition of Brucella abortus local isolate. The B. abortus isolate is a collection of Department of Agriculture, Research and Technology Center for Isotopes and Radiation Application-BRIN), isolated from the hygroma fluid in the case field. N-Lauroylsarcosine (Sarkosyl) methods used to extract outer membrane bacteria. OMP B. abortus was measured protein molecular weight by SDS-PAGE and standard molecular weight of 10-250 kDa. This study showed that the profile Outer membrane Proteins (OMP) Brucella abortus local isolate had eight bands there were 16.7-kDa, 18-kDa, 26.6-kDa, 33-kDa, 42-kDa, 55-kDa, 72-kDa, and 91.3-kD.