Kinetic analysis of ribozyme reactions is a common method to evaluate and compare activities of catalytic RNAs. The hairpin ribozyme catalyzes the reversible cleavage of a suitable RNA substrate at a specific site. Hairpin ribozyme variants as an allosteric ribozyme responsive to flavine mononucleotide and a hairpin-derived twin ribozyme that catalyzes two cleavage reactions and two ligation events with the result of a fragment exchange have been developed by rational design and were kinetically characterized. Herein, protocols for preparation of ribozymes and dye-labeled substrates as well as for analysis of cleavage, ligation, and fragment exchange reactions are provided. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Appel, B., Marschall, T., Strahl, A., & Müller, S. (2012). Kinetic characterization of hairpin ribozyme variants. Methods in Molecular Biology, 848, 41–59. https://doi.org/10.1007/978-1-61779-545-9_4
Mendeley helps you to discover research relevant for your work.