Effect of ischemia and reperfusion on cardiac ryanodine receptors - Sarcoplasmic reticulum Ca2+ channels

Abstract

We investigated the effect of ischemia and reperfusion on the cardiac ryanodine receptor, which corresponds to the sarcoplasmic reticulum Ca2+ channel. Isolated working rat hearts were subjected to 10 to 30 minutes of global ischemia, followed or not by reperfusion. Ischemia produced significant reduction in the density of high-affinity 3H-ryanodine binding sites, determined either in whole-heart homogenate (Bmax, 220±22, 203±12, and 228±14 fmol/mg protein after 10, 20, and 30 minutes of ischemia versus 298±18 fmol/mg protein in the control condition; P