Two new bifunctional reagents suited for the step-wise cross-linking of cysteine and lysine residues in proteins are described. Application to lactate dehydrogenase yields a cross-link between cysteine-165 and lysine-179, which suggests an alternative mechanism by which the "essential" cysteine reacts. For the mapping of the environment of a known and well defined amino acid the use of semireversible bifunctional reagents is suggested.
CITATION STYLE
Trommer, W. E., Friebel, K., Kiltz, H. H., & Kolkenbrock, H. J. (1977). Synthesis and application of new bifunctional reagents. Advances in Experimental Medicine and Biology, 86 A, 187–195. https://doi.org/10.1007/978-1-4684-3282-4_10
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