Effects of cations, polyamines and other aminoglycosides on gentamicin C2 binding to ribosomes from sensitive and resistant escherichia coli strains

Abstract

Gentamicin C2 interacts cooperatively with ribosomes from a sensitive Escherichia coli strain in a multiphasic way with several classes of sites. It is shown that this binding is highlydependent on Mg++ and natural endogenous polyamine concentrations. The differences observed between ribosomes from sensitive and resistant strains may be explained by the absence of specific cooperative gentamicin interactions with resistant ribosomes. The effects of other aminoglycoside antibiotics are discussed in terms of structure-activity relationships. © 1986, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.