A germin-like protein gene of rice increased superoxide dismutase activity in transformed tobacco

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Abstract

Germin and germin-like proteins (GLPs) are a broad and diverse family of developmentally regulated proteins widely distributed in plants. Oryza sativa L. harbours a large family of GLPs and serves as a good model for their study. In the present study, a germin-like protein gene (OsRGLP1) of rice origin was characterized by its heterologous expression in tobacco. The real-time PCR established almost a uniform expression of OsRGLP1 in leaves, stem, and roots of T1Nicotiana tabacum cv. Samsun. Although no morphological difference was apparent between T0 transgenic and wild-type plants, leaves of mature transgenic plants showed necrotic lesions associated with an elevated content of H2O2, which was evidenced by in situ 3,3′-diaminobenzidine staining. A significantly higher activity of heat resistant superoxide dismutase (SOD) was observed in the transgenic plants as compared to the wild-type. The SOD activity in the transgenic plants was insensitive to potassium cyanide and sensitive to H2O2.

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Yasmin, T., Mumtaz, A., Mahmood, T., Hyder, M. Z., & Naqvi, S. M. S. (2015). A germin-like protein gene of rice increased superoxide dismutase activity in transformed tobacco. Biologia Plantarum, 59(3), 456–462. https://doi.org/10.1007/s10535-015-0524-z

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