In-vitro antioxidant and in-vivo hepatoprotective activity of ethenolic extract of Tectona grandis bark against CCl4 induced liver injury in rats

Abstract

Objectives: The systematic screening of Tectona grandis bark with the purpose of discovering new bioactive compounds as a hepatoprotective agent and to establish the scientific basis for the therapeutic actions of traditional plant medicines. Methods: Tectona grandis bark ethenolic extract was studied for the hepatoprotective activity against CCl4 induced liver injury in rats. Serum enzymes level, total bilirubin and histopathological study of liver were performed. This extract’s DPPH radical scavenging potential was also studied. Results: Oral administration of ethenolic extract of Tectona grandis bark (200 mg/kg) exhibited significant reduction (p<0.05) in CCl4-induced increased levels of SGPT, SGOT, ALP and bilirubin (Total) concentration. Treatment with Liv 52 syrup also reversed the hepatotoxicity significantly (p<0.05). Histopathological studies also provided supportive evidence for biochemical analysis. This extract also showed better activity in quenching DPPH radical. Conclusion: Tectona grandis bark ethenolic extract shown to have hepatoprotective and antioxidant action due to presence of quinones and tannin like phytoconstituents.