Locked nucleic acid (LNA) is a nucleic acid analog containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA-mimicking sugar conformation. This conformational restriction is translated into unprecedented hybridization affinity towards complementary single-stranded RNA molecules. That makes fully modified LNAs, LNA/DNA mixmers, or LNA/RNA mixmers uniquely suited for mimicking RNA structures and for RNA targeting in vitro or in vivo. The focus of this chapter is on LNA antisense, LNA-modified DNAzymes (LNAzymes), LNA-modified small interfering (si)RNA (siLNA), LNA-enhanced expression profiling by real-time RT-PCR and detection and analysis of microRNAs by LNA-modified probes. © 2006 Springer-Verlag Berlin Heidelberg.
CITATION STYLE
Kauppinen, S., Vester, B., & Wengel, J. (2006). Locked nucleic acid: High-affinity targeting of complementary RNA for RNomics. Handbook of Experimental Pharmacology, 173, 405–422. https://doi.org/10.1007/3-540-27262-3_21
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