Supercritical Fluid Extraction and Chromatographic Analysis (HRGC-FID and HRGC-MS) of Lupinus spp. Alkaloids

Abstract

The alkaloid extracts from Lupinus spp., obtained by conventional methods (maceration/sonication - solid phase extraction; maceration/sonication - liquid-liquid extraction) and SFE (supercritical fluid extraction) using CO2 and modified CO2 (CO2/MeOH, CO2/EtOH, CO2/iPrOH and CO2/ H2O) were analysed by HRGC-FID (high resolution gas chromatography - flame ionization detector) and HRGC-MS (high resolution gas chromatography - mass spectrometry). The HRGC-FID quantitative analyses were performed with an internal standard method for quantification of lupanine, multiflorine and a spartein-like alkaloid. HRGC-MS allowed identification of the chemical constituents (alkaloids and other compounds) from these extracts.