The apoplastic fluid moving in the extracellular space external to the plasma membrane provides a means of delivering molecules and facilitates intercellular communications. However, the apoplastic fluid extraction from in planta systems remains challenging and this is particularly true for grapevine (Vitis vinifera L.), a worldwide-cultivated fruit plant. Here, we describe an optimized vacuum-infiltration-centrifugation method to extract soluble proteins from apoplastic fluid of grapevine leaves. This optimized method allows recovering of the grapevine apoplastic soluble proteins suitable for mono- and bi-dimensional gel electrophoresis for further proteomic analysis in order to elucidate their physiological functions.
CITATION STYLE
Delaunois, B., Baillieul, F., Clément, C., Jeandet, P., & Cordelier, S. (2016). Vacuum infiltration-centrifugation method for apoplastic protein extraction in grapevine. In Methods in Molecular Biology (Vol. 1459, pp. 249–257). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3804-9_17
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