A review: Applications of flow cytometry in immunohematology

Abstract

Flow cytometry has been extensively used in lymphocyte subset analysis. It has also been used in specialized studies of tumors,(1,2) hematopoietic stem cells, (3) virus-specific antigens,4 and to monitor monoclonal antibody production.sup(5) Many blood bankers see the potential of flow cytometry in immunohematological problem solving. This article reviews publications that have used flow cytometry for quantitation of red blood cell (RBC)-boundfluorescein molecules, (6) measurement of RBC-bound IgG in patients with hemolytic anenmia, (7,8) analysis of RBC antigen site density, (9-13) and detection of minor cell population, (14-19) fetal RBCs,sup(20-22) and platelet antibodies.(23,24).