Freeze-drying of proteins

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Abstract

Freeze-drying has become one of the most important processes for the preservation of biological products. This chapter provides protocols for freeze-drying of proteins and discusses the importance of formulation, cycle development, and validation. Specific formulations for stabilization of proteins are presented as well as advice on common problems with freeze-drying of proteins.

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Liu, B., & Zhou, X. (2021). Freeze-drying of proteins. In Methods in Molecular Biology (Vol. 2180, pp. 683–702). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0783-1_37

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