RNA‐binding properties of hnRNP proteins

Abstract

The RNA‐binding properties of the hnRNP monoparticle proteins were examined using a renaturing blotting procedure. All ‘core’ proteins are able to bind single‐stranded nucleic acids, probably not sequence‐specific. The core proteins C1 and, in one case A2 and B2, are able to bind nucleic acids which are double‐stranded or which show a high degree of base‐paired regions, among them U1 snRNA, whereas A1, B1 and C2 are unable to bind base‐paired nucleic acids. The characteristics of C1 in binding base‐paired nucleic acids are especially interesting, since the involvement of C1 in the splicing process has been described. Copyright © 1988, Wiley Blackwell. All rights reserved