Synthesis, characterization, antioxidant activity, and toxicity properties of tripolyphosphate crosslinked chitosan

Abstract

Chitosan is a biopolymer obtained from chitin deacetylation reaction. Chitin is a constituent of shrimp outer shell. Chitosan modification using a crosslinker agent could increase the active groups and stabilize the physical character. One of the non-toxic crosslink agents was tripolyphosphate. This study aimed at synthesizing tripolyphosphate crosslinked chitosan (Cs-TPP) to test the antioxidant and toxicity. Synthesizing tripolyphosphate chitosan was conducted using ionic gelation method. The antioxidant activity test was studied from DPPH (1,1-diphenyl-2-pikrilhidrazil) radicals scavenging and the ability to inhibit linoleic acid oxidation. The toxicity test was using Brine Shrimp Lethality Test method. The result showed that Cs-TPP could be synthesized from pink shrimp and showed antioxidant activity. IC50 value of Cs-TPP is 0.246 mg/mL. The activity was due to amino and hydroxyl groups. The BSLT method showed that Cs-TPP had the potential to be applied for antibacterial, antivirus, or anticancer products.