Adenovirus are important pathogen primarily associated to respiratory infections of children and military personnel, even though it is also associated to cases of conjunctivitis and keratoconjunctivitis. We analyzed respiratory secretion collected from subjects with and without respiratory infection symptoms, being 181 civilians and 221 military subjects. The samples were inoculated in HEp-2 and/or A549 tissue cultures for viral isolation. Samples presenting cytopathogenic effect (CPE) in any tissue culture were tested by a polymerase chain reaction (PCR) assay to confirm adenovirus isolation. The isolates confirmed as adenovirus were further analyzed by restriction endonuclease assay for determination of viral species. Three isolates were identified as specie A (two from civilian and one from military), one isolate from military was identified as specie C, and one isolate from civilian was identified as specie D. For two isolates the specie could not be identified.
CITATION STYLE
De Albuquerque, M. C. M., Da Silva, F. M., Soares, C. C., Volotão, E. de M., & Santos, N. (2003). Adenoviruses isolated from civilian and military personnel in the city of Rio de Janeiro, Brazil. Revista Do Instituto de Medicina Tropical de Sao Paulo, 45(4), 233–236. https://doi.org/10.1590/s0036-46652003000400012
Mendeley helps you to discover research relevant for your work.