Extracellular vesicles, such as exosomes and microvesicles, have been shown to contain potential microRNA (miRNA) biomarkers that may be utilized in the diagnosis of various diseases from cancer to neurological disorders. The unique nature of the extracellular vesicle bilayer allows miRNA to be protected from degradation making it an ideal source of material for biomarkers discovery from both fresh and archived samples. Here we describe the quantitative analysis of miRNA isolated from exosomes by quantitative PCR and digital PCR.
CITATION STYLE
Bellingham, S. A., Shambrook, M., & Hill, A. F. (2017). Quantitative analysis of exosomal miRNA via qPCR and digital PCR. In Methods in Molecular Biology (Vol. 1545, pp. 55–70). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6728-5_5
Mendeley helps you to discover research relevant for your work.