With the renewed enthusiasm in immuno-oncology, characterization of the tumor immune microenvironment constitutes an essential and unique aspect to the assessment of therapeutics. The isolation of tumor-infiltrating lymphocytes (TILs) is a desirable approach toward the understanding of antitumor immune response. This chapter provides an effective protocol to mechanically dissociate tumor tissue and generate single-cell suspension from excised tumors. TILs are then isolated by Ficoll-Paque density gradient centrifugation. This protocol is applicable to both human and experimental tumors in immunocompetent murine models.
CITATION STYLE
Tan, Y. S., & Lei, Y. L. (2019). Isolation of tumor-infiltrating lymphocytes by ficoll-paque density gradient centrifugation. In Methods in Molecular Biology (Vol. 1960, pp. 93–99). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9167-9_8
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