Induction of chromosomal translocations in human cells is of a great interest to study tumorigenesis and genome instability. Here, we explain in detail a method to induce translocations using the transcription activator-like effector nucleases (TALENs). We describe how to detect translocation formation by PCR, calculate translocation frequency by 96-well PCR screen, and analyze breakpoint junctions. When inducing cancer translocations, it is also possible to detect the fusion gene by FISH analysis or western blot
CITATION STYLE
Piganeau, M., Renouf, B., Ghezraoui, H., & Brunet, E. (2016). TALEN-induced translocations in human cells. In Methods in Molecular Biology (Vol. 1338, pp. 99–117). Humana Press Inc. https://doi.org/10.1007/978-1-4939-2932-0_9
Mendeley helps you to discover research relevant for your work.