To select antisense oligonucleotides (AONs) that specifically target the genes of rat organic anion transporting polypeptide (oatp) subtypes, in vitro selection and an alignment program were used. When we incorporated a couple of our original 2'-O,4'-C-ethylene nucleoside (ENA) residues into the AONs at both the 3' and 5' ends, the inhibitory activity of these oatp subtype-specific AONs was enhanced. This strategy of combining in vitro selection with the use of an alignment program as well as incorporating ENA residues, was effective in synthesizing oatp subtype-specific AONs.
CITATION STYLE
Takagi, M., Morita, K., Nakai, D., Nakagomi, R., Tokui, T., & Koizumi, M. (2003). Design and synthesis of oatp subtype-specific antisense oligonucleotides having 2’-O,4’-C-ethylene-bridged nucleic acids (ENA). Nucleic Acids Research. Supplement (2001), (3), 83–84. https://doi.org/10.1093/nass/3.1.83
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