Evaluation of Aerobic Oral Microbial Flora Pattern in Necrotic Pulp with Chronic Periapical Abscess and Oral Mucosa

Abstract

The most common type of dental abscess is a periapical abscess. It has been shown that endodontic infection comprises of a complex mixture of bacterial species. Fungi are oral commensal and causes wide variety of infections in human beings. The aim of this study was to identify species of the genus Candida and various aerobic bacterial species in clinically symptomatic patients having pulp necrosis with chronic endodontic periapical abscess, with radiographic images. The study included 75 patients of both sexes aged 19- 75 years. Samples were taken from root canals with sterile # 25 paper points and from oral mucosa with a sterile swab. In chronic periapical abscess, various organisms grown were identified Candida species (37.33%), Streptococcus viridans (9.33%), Non hemolytic Streptococcus (5.3%),S. pyogenes (1.3%), Staphylococcus aureus (8%),Gram positive bacilli (8%) & Coagulase negative Staphylococcus (5.33%). Among Candida, seven different species were identified (C. albicans (24%), C. tropicalis (5.33%), C. krusei (2.66%), C. glabrata (1.33%), C. guilliermondii (1.3%), C. parapsilopsis (1.3%), and C. pseudotropicalis (1.33%). In oral mucosa Candida species (54.66%) was maximally isolated followed by Streptococcus viridans 14(22.66%). Considering all the samples isolated from oral mucosa, there was a significantly greater frequency of C. albicans, S. viridans than periapical zone of necrotic canals. Introduction