Preparative HPLC (prep-HPLC) has emerged as a mainstay of natural products isolation and purification. The various modes available to date, e.g. normal phase, reversed-phase, size exclusion, and ion exchange, can be used to purify most classes of natural products. This chapter presents an overview of the different modes along with a practical guide as to how to purify a natural product using the most robust and widely used of the modes, namely reversed-phase prep-HPLC. Instrumentation set-up and detection methods, sample preparation, method development, and sample work up are also discussed. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Latif, Z., & Sarker, S. D. (2012). Isolation of natural products by preparative high performance liquid chromatography (Prep-HPLC). Methods in Molecular Biology, 864, 255–274. https://doi.org/10.1007/978-1-61779-624-1_10
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