Preparation of Bacterial Samples for 2-D PAGE

Abstract

Sample preparation is a very crucial step in two-dimensional (2-D) gel electrophoresis, in which the proteins of the sample must be brought into a state where they can be separated by isoelectric focusing in the first dimension. That is, they must be denatured, reduced, and solubilized, and they must be kept so during electrophoresis without changing their pI. The sample buffer for this purpose is traditionally called the lysis buffer.