Survei Polimorfisme Tetua untuk Pengembangan Panel CSSL Padi (Oryza sativa L.) dan Identifikasi Tanaman F1

Abstract

Raising yield potential of modern indica varieties is essential to meet the increased demand of rice production. This is due to increased human population, threats of climate change and degradation of agricultural resources. The use of chromosome segment substitution lines (CSSL) is more effective for identification of genes those are useful for improvement of yield potential. The aims of this study were to observe the morphological trait differences between recipient parent (var. Ciherang) and three candidates of donor parent (var. Fatmawati and new plant type lines, i.e. B12743 and B11143D), to identify polymorphic SSR markers among them and to verify F1 individuals. Ciherang and B11143D showed significant differences on flowering time, plant height, flag leaf area, tiller number, productive tiller number, panicle length, spikelet number per panicle and 1,000 grain weight. The rate of SSR marker polymorphisms between Ciherang and B11143D was the highest, where 155 of 513 markers (30.2%) were polymorphic. Marker genotyping using three polymorphic markers showed that 26 of 27 plants resulted from the cross of Ciherang х B11143D were F1. These F1 plants could become the basis of CSSL panel that facilitate the mapping of genes responsible for increasing the yield potential.