Target site resistance to acetolactate synthase inhibitors in a fall panicum (Panicum dichotomiflorum Michx.) accession from Wisconsin and its response to alternative herbicides

Abstract

Owing to the lack of effective POST herbicide options, producers typically rely on nicosulfuron as the main POST grass herbicide in sweet corn production systems. In 2019, a Wisconsin sweet corn producer reported fall panicum control escapes after spraying nicosulfuron. Seeds from mature plants were collected to (1) measure fall panicum response to acetolactate synthase (ALS)-inhibiting herbicides, (2) elucidate the resistance mechanism, and (3) evaluate its response to alternative POST herbicides. Greenhouse and laboratory investigations were conducted to assess fall panicum response to ALS-inhibiting herbicides and elucidate the resistance mechanism. Dose-response results showed that fall panicum was highly resistant to nicosulfuron with a resistance ratio of >12.9-fold (survived rates >254 g ai ha-1, or 8× the field label rate). Molecular and genetic studies indicated that there are multiple ALS gene copies in fall panicum and that resistance was due to a mutation in one copy, resulting in an Asp-376-Glu amino acid substitution. Additional greenhouse experiments indicate that clethodim (105 g ai ha-1), quizalofop-p-ethyl (70 g ae ha-1), glyphosate (864 g ae ha-1), and glufosinate (650 g ai ha-1) are effective POST options to manage the ALS-resistant fall panicum (>90.0% control and 96.8% biomass reduction) in rotational years. The 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides isoxaflutole (105 g ai ha-1), mesotrione (105 g ai ha-1), tembotrione (92 g ai ha-1), and tolpyralate (39 g ai ha-1) did not provide effective POST fall panicum control. Because these herbicides are commonly used for POST weed control in sweet corn, more investigations are required to evaluate combinations of HPPD-inhibiting herbicides with herbicides from other sites of action for POST fall panicum control. Herein we confirm the first case of herbicide resistance in fall panicum in the United States.