Genetically encoded biosensors utilizing the Förster resonance energy transfer (FRET) are powerful tools for live cell imaging of various cellular processes. Our group has previously developed a series of FRET-based biosensors, named “ATeam,” for visualization of ATP levels inside a single living cell. ATeam not only provides a window of insight into a single cell but also allows for visualization of ATP levels in mitochondrial matrix of a single living cell. This novel tool is able to monitor alterations in cellular ATP in response to various treatments in real time. Here we present a method for the evaluation of ATP levels in mitochondria in living cells by using ATeam. At the end of this chapter, an example of experimental results is described for a better understanding of the presented procedure.
CITATION STYLE
Yoshida, T., Alfaqaan, S., Sasaoka, N., & Imamura, H. (2017). Application of FRET-based biosensor “ATeam” for visualization of ATP levels in the mitochondrial matrix of living mammalian cells. In Methods in Molecular Biology (Vol. 1567, pp. 231–243). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6824-4_14
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