Vaccine Efficacy of Bm86 Ortholog of H. a. anatolicum , rHaa86 Expressed in Prokaryotic Expression System

Abstract

The use of tick vaccine in controlling ticks and tick borne diseases has been proved effective in integrated tick management format. For the control of H. a. anatolicum , Bm86 ortholog of H. a. anatolicum was cloned and expressed as fusion protein in E. coli as E. coli -pETHaa86. The molecular weight of the rHaa86 was 97 kDa with a 19 kDa fusion tag of thioredoxin protein. The expressed protein was characterized immunologically and vaccine efficacy was evaluated. After 120 hours of challenge, only 26% tick could successfully fed on immunized animals. Besides significant reduction in feeding percentages, a significant reduction of 49.6 mg; P